In-House Extracted Soybean Protein Can Reduce the Enzyme Dosage in Biomass Saccharification

Bioconversion of the complex carbohydrates present in lignocellulosic biomass into simple sugars, order to obtain biofuels and bio-based products, is still limited by low performance enzymatic saccharification reaction high cost cellulolytic enzymes. Low-cost additives such as soybean protein can reduce unproductive adsorption cellulases onto lignin, increasing conversion rates reducing enzyme losses. Here, investigation was made effects different fractions, extracted in-house, on hydrothermally pretreated sugarcane bagasse. The glucose released during increased up 76% presence in-house protein, compared control (without additive). A remarkable finding that technique allowed loading be decreased four-fold. results suggested alkali-extracted proteins presented surface hydrophobicity, which enhanced their interaction with lignin reduced binding cellulases. Among main glycinin had best effect improving saccharification, could have been due its higher hydrophobicity. Hence, seem interesting alternative capable efficiency future biorefineries.